README for Figure S2.csv 
*** This file contains the raw data obtained on DNA, DNA-Rok, DNA-sRok and DNA-Rok-sRok complexes using bridging assay experiments represented in Figure S2 of 
Article: The B. subtilis Rok protein is an atypical H-NS-like protein irresponsive to physico-chemical cues
Authors: Erkelens, Qin, van Erp, Miguel-Arribas, Abia, Keek, Markus, Cajili, Schwab, Meijer and Dame
Journal:  
DOI:
Corresponding author: rtdame@chem.leidenuniv.nl; wmeijer@cbm.csic.es


Legend Figure S2:Rok bridges dsDNA but not ssDNA DNA recovery (%) as a function of the length of prey DNA in bp 
in the presence of 0.27 M Rok with 50 mM KCl at 25C. The bait DNA was the 685 bp dsDNA as used for the other 
bridging experiments. Data are plotted as mean values and the error bars represent the standard deviation from 
three independent measurements.

*** The data were obtained using bridging assay experiments as described in the associated article. 

***The data labeled overview represents the values plotted in the graph of figure S2. The raw data contains the individual replicates.
Column A: Sample number
Column B: Presence of bait DNA in sample
Column C: Presence of 32P-labeled prey DNA in sample
Column D: Amount of Rok in the sample in micromolar
Column E: Characteristics of prey DNA (length in basepairs and double/single-stranded)
Column F: Counts per minutes 
Column G: DNA recovery (%)

Columns B, C, D and E indicate the contents of the sample. Column F indicates the radioactivity of the sample. 
Column G indicates the DNA recovery, which is calculated by subtracting the value in column F of the control 
(sample minus bait DNA) from the sample itself. This value is then divided by the values in column F of sample 1 
and multiplied by 100%. 









